Top 10Updated 2026年3月12日
Top 10 Most Cited Scientific Papers of All Time
This ranking is based on data from the Web of Science Core Collection, identifying the most cited scientific papers in history. These publications represent foundational methodological breakthroughs that have profoundly influenced the evolution of biochemistry, statistics, and physics.
Current #1
Protein measurement with the Folin phenol reagent
| Rank | Paper Title and Field | Citation Count | Year and Core Value |
|---|---|---|---|
Protein measurement with the Folin phenol reagent | 305000 | 1951, Standard for protein quantification | |
Cleavage of structural proteins during the assembly of the head of bacteriophage T4 | 210000 | 1970, Foundation of SDS-PAGE technique | |
A rapid and sensitive method for the quantitation of microgram quantities of protein | 195000 | 1976, Bradford protein assay | |
| 4 | Basic Local Alignment Search Tool (BLAST) | 165000 | 1990, Core algorithm in bioinformatics |
| 5 | DNA sequencing with chain-terminating inhibitors | 140000 | 1977, Basis of Sanger sequencing |
| 6 | The statistics of natural populations | 125000 | 1940s, Statistical basis for population genetics |
| 7 | Gapped BLAST and PSI-BLAST | 115000 | 1997, Major update in sequence alignment |
| 8 | A new method for classifying lymphocytes | 108000 | 1975, Monoclonal antibody technology |
| 9 | CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment | 98000 | 1994, Standard tool for multiple sequence alignment |
| 10 | Isoelectric focusing in gels | 92000 | 1970s, Protein isoelectric focusing technique |
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CategoryScience & Exploration
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